Accumulation of free radicals, coupled with an increase in oxidative stress, has been implicated in the pathogenesis of several disease states and in the mechanism of action for toxicity of many compounds. This protocol can be used to measure these toxicity endpoints. The OxiSelect™ In Vitro ROS / RNS Assay Kit allows for the measurement of the total amount of free radicals in cell effluent by using a specific ROS / RNS fluorogenic probe. The fluorescence intensity is proportional to the total ROS / RNS levels within the sample.
This protocol uses the Proximal Tubule Kidney-Chip as a reference point. These methods and assay conditions could change with different Organ-Chips.