Introduction
Alkaline phosphatases (ALPs) are a family of cell surface glycoproteins with ALP isoenzymes expressed in kidney as well as in a variety of other organs such as liver, intestine, and bone. In the clinic, elevated levels of serum ALP are associated with disease or injury in serval organs. It can be applied as a marker of kidney injury and disease. This protocol uses the Proximal Tubule Kidney-Chip as a reference point, and these assay conditions could change with a different Organ-Chip.