Live Staining of CLF Uptake into Bile Canaliculi

Introduction

CorningĀ® Cholyl-lysyl-fluorescein (CLF) staining is used to visualize the structure and function of bile canaliculi in polarized hepatocytes. CLF is a substrate for the canalicular bile salt export pump (BSEP), thus it can be used to visualize BSEP-mediated canalicular efflux, as well as to label the bile canaliculi structures.